In this Perspective, the authors summarize the current knowledge on the post-COVID-19 condition, focusing on the neurological manifestations, and discuss the applicability of existing animal models to recapitulate human condition.

Poor replicability in animal research can be a result of low external validity driven by rigorous standardization of study populations. This study investigates how heterogenization of study populations by using mice from different breeding sites might affect the replicability of animal studies conducted in a single facility. The findings suggest that heterogenization by breeding site has limited capacity to improve replicability in animal research.

The 3R principles provide an ethical framework for animal research throughout the world. However, despite the increasing awareness of these principles, there is still a lot of room for improving their implementation, especially when it comes to reduction. By combining Bayesian statistics with a shift in experimental design, here we present an entirely new idea to reduce animal numbers within experiments.

The authors compare the efficacy of injectable and oral broad-spectrum antibiotics on reducing bacteria from the upper respiratory tract of ferrets, before evaluating the effects of commensal bacteria reduction on influenza A virus infection.

The authors evaluate multiple factors to improve knock-in rates using CRISPR-mediated homology-directed repair in zebrafish, before demonstrating that prime editing is a superior alternative.

This Article presents the results of a global multi-laboratory study assessing the effects of housing conditions and experimental settings on novel tank test responses in adult zebrafish.

This study, which establishes reference intervals for biochemical and hematological parameters in healthy macaques and describes changes in these parameters after vaccination, will facilitate toxicity monitoring in preclinical vaccine studies.

This proof-of-concept study for the intranasal delivery of neuroactive drugs in adult zebrafish introduces a method that enables fast delivery of drugs such as arecoline and nicotine to the brain while requiring smaller quantities than water immersion methods.

This article describes a computational model to forecast population dynamics in a laboratory colony of common marmosets. The model can be used to predict future animal stock levels and support colony management.