In this Perspective, the authors summarize the current knowledge on the post-COVID-19 condition, focusing on the neurological manifestations, and discuss the applicability of existing animal models to recapitulate human condition.

Poor replicability in animal research can be a result of low external validity driven by rigorous standardization of study populations. This study investigates how heterogenization of study populations by using mice from different breeding sites might affect the replicability of animal studies conducted in a single facility. The findings suggest that heterogenization by breeding site has limited capacity to improve replicability in animal research.

The 3R principles provide an ethical framework for animal research throughout the world. However, despite the increasing awareness of these principles, there is still a lot of room for improving their implementation, especially when it comes to reduction. By combining Bayesian statistics with a shift in experimental design, here we present an entirely new idea to reduce animal numbers within experiments.

The authors compare muscle function, histology and molecular markers, and biochemical plasma profiles in male and female HSA^LR mice to identify potential sex differences in this mouse model of myotonic dystrophy type I.

Wound healing assays are commonly used to study tissue repair in mice. The authors show that wound localization and housing conditions are critical factors that can affect the reliability and reproducibility of the results of this assay.

This study explores the use of electrical stunning as a viable alternative to anesthetic overdose and rapid cooling for euthanizing zebrafish larvae, potentially improving the welfare of over 5 million zebrafish in biomedical research.

Differences in standard chow diets can influence mouse model phenotypes and impact experimental reproducibility. Here Knuth et al. show that the timing during which mice are exposed to standard chow can also affect phenotypic outcomes.

The marble burying task (MBT) is used to measure anxiety-like behavior, relying on a snapshot of the number of marbles buried at the end of the test. This Brief Communication introduces a more extensive behavioral analysis to improve the translational value of MBT for clinical psychological phenotypes.

This Protocol describes a minimally invasive technique for central nervous system drug delivery in rodents. The procedure allows the use of the transnasal route to deliver blood–brain barrier-impermeant drugs.